On the functional role of the Tyr‐639 residue of bacteriophage T7 RNA polymerase
- 20 July 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 306 (2-3) , 129-132
- https://doi.org/10.1016/0014-5793(92)80983-n
Abstract
Substitution or Asp for a Tyr residue normally present at position 639 of the bacteriophage T7 RNA polymerase leads to a drastic drop in the enzymatic activity. This mutation does not affect the enzyme‐promoter interaction but decreases the ability of the RNA polymerase to discriminate between GTP and ATP molecules, resulting in a decrease in the rate of the incorporation of the nucleotide into the RNA chain.Keywords
This publication has 11 references indexed in Scilit:
- Lys631 residue in the active site of the bacteriophage T7 RNA polymerase. Affinity labeling and site-directed mutagenesisEuropean Journal of Biochemistry, 1991
- Inactivation of bacteriophage T7 DNA‐dependent RNA polymerase by 5′‐p‐fluorosulfonylbenzoyladenosineEuropean Journal of Biochemistry, 1990
- The gene for Klebsiella bacteriophage K11 RNA polymerase: Sequence and comparison with the homologous genes of phages T7, T3, and SP6Molecular Genetics and Genomics, 1990
- Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7Cell, 1987
- Nucleotide sequence and expression of the cloned gene of bacteriphage SP6 RNA polymeraseNucleic Acids Research, 1987
- Sequence and analysis of the gene for bacteriophage T3 RNA polymeraseNucleic Acids Research, 1985
- 4 Bacteriophage DNA-Dependent RNA PolymerasesPublished by Elsevier ,1982
- Nucleotide sequence of the cloned gene for bacteriophage T7 RNA polymeraseJournal of Molecular Biology, 1981
- The nucleotide sequence of gene 1 of T7 phage DNA which codes for the phage‐specific DNA‐dependent RNA‐polymeraseFEBS Letters, 1981
- Fluorescent modification of adenosine-containing coenzymes. Biological activities and spectroscopic propertiesBiochemistry, 1972