Evaluation of the Mastalex latex agglutination test for methicillin resistance in Staphylococcus aureus grown on different screening media
Open Access
- 1 February 2001
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 47 (2) , 187-189
- https://doi.org/10.1093/jac/47.2.187
Abstract
The Mastalex MRSA latex agglutination method was evaluated with 52 methicillin-resistant and 27 methicillin-susceptible strains of Staphylococcus aureus grown on various media. All tests were correct with colonies grown on blood agar with or without oxacillin 2 mg/L. Tests on colonies grown on mannitol salt agar were less reliable, although addition of oxacillin 2 mg/L improved performance. One of the 26 MRSA which grew on Baird–Parker medium with 8 mg/L ciprofloxacin gave a false-negative result. Agglutination was faster when strains were grown on media with oxacillin. The method would be particularly useful for urgent confirmation of resistance.Keywords
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