Development of a Rat Urinary Kallikrein-Binding Radioimmunoassay and Identification of Homologous Enzyme in Plasma

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Abstract
A complete description of a component-binding radioimmunoassay [RIA] of rat urinary kallikrein is presented. Each step of the RIA was evaluated and optimized. The problems encountered in the development of the assay are discussed; this may be a guide to the treatment of similar problems in the development of a comparable component-binding RIA. Protocols are presented for performance of the assay with [3H]-labeled or [125I]-labeled rat urinary kallikrein. RIA and immuno-double-diffusion data suggested that rat plasma indeed contains an antigen similar to glandular kallikrein. However, quantitation of the antigen by the direct RIA must await appropriate developments for efficient dissociation and/or denaturation of interfering substances.