SLOW REACTING SUBSTANCE (SRS) FROM IONOPHORE A23187-STIMULATED HUMAN-LEUKEMIC BASOPHILS .1. EVIDENCE FOR A PRECURSOR ROLE OF ARACHIDONIC-ACID AND INITIAL PURIFICATION

  • 1 January 1979
    • journal article
    • research article
    • Vol. 123  (6) , 2814-2816
Abstract
SRS [slow-reacting substance] was generated from human leukemic basophils upon stimulation with ionophore A23187. Radiolabel from [14C]-AA was incorporated into SRS with continued co-migration of radioactivity and bioactivity through several chromatographic systems including DEAE-cellulose, silicic acid and RP-HPLC [reverse phase high pressure liquid chromatography]. Human basophilic leukemia SRS displayed physiochemical properties similar to those of rat basophilic leukemia cell SRS.