Secretion by a Hybridoma of Antibodies against Human α-Fetoprotein

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Abstract
To the Editor: Advances in somatic-cell hybridization have made it possible to produce monoclonal antibodies in tissue culture.1 Immunization of mice with pertinent immunogens stimulates the production of antibodies specific for the immunogens. Spleen cells, which include specific antibody-producing lymphocytes, can be explanted and fused with mouse myeloma-cell lines2 to derive permanent cell lines (hybridomas) that produce antibodies of predefined specificities. With appropriate cloning techniques, a monoclonal antibody-producing hybridoma can be isolated from a mixture of hybrid cells in the tissue culture. Monoclonal antibodies specific for red cells, haptens, proteins, carbohydrate, viruses, and major histocompatibility antigens have recently been produced . . .