Phosphodiesterase specific inhibitors control cell growth of a human neuroepithelioma cell line

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Abstract
The effects of cyclic nucleotide phosphodiesterase (PDE) inhibitors on cell proliferation of SK-N-MC human neuroepithelioma cell line was studied. Clonal density experiments in the presence of 100 μM rolipram and zaprinast showed respectively 27% and 91% inhibition. The effects of PDE inhibitors were then investigated on crude cell extracts; the calculated IC50 were 32 μM for zaprinast and 16 nM for DC-TA-46; the latter inhibitor was used instead of rolipram for itshigher efficacy. Dose-response experiments in clonal density conditions showed IC50 of 5 μM and 1.8 μM in the presence respectively of zaprinast and rolipram. These data show that both inhibitors are effective in reducing cell growth, although the response was quantitatively different.