The isolation of barley-aleurone protoplasts
- 1 January 1971
- journal article
- Published by Springer Nature in Planta
- Vol. 101 (2) , 95-100
- https://doi.org/10.1007/bf00387620
Abstract
A procedure is described for the isolation of large numbers of viable aleurone protoplasts. After treatment with “Onozuka” cellulase protoplasts were obtained which were surrounded by a thin, “Onozuka”-resistant wall. These cells were termed “spheroplasts”. Treatment of spheroplasts with “Glusulase” digested away the residual wall, yielding naked protoplasts. Measurements of respiration using a Clarke-type oxygen electrode indicated that aleurone cells isolated by this procedure were viable.Keywords
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