Abstract
Temperature was found to have a marked effect upon the generation times of Amoeba proteus when cultured with Tetrahymena pyriformis at 20, 15, and 10 °C. The length of the cell cycle varied from 44 h at 20 °C to 2926 h at 10 °C. For each temperature studied, generation times followed a U-shaped distribution where the greatest division potential was determined by the prey concentration. Optimum reproductive rates were achieved at lower food levels as temperature was decreased. The results are discussed in the context of the available published literature.

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