APPARENT TRANS CONTROL OF MURINE βGLUCURONIDASE SYNTHESIS BY A TEMPORAL GENETIC ELEMENT
- 10 December 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 90 (4) , 725-734
- https://doi.org/10.1093/genetics/90.4.725
Abstract
A difference in the heat-inactivation kinetics between the β-glucuronidases of C3HeB/FeJ and C57Bl/6J mice was utilized to assess the mode of action of a temporal genetic element in controlling the expression of the β-glucuronidase structural gene Gus. The heat-inactivation kinetics of liver and kidney β-glucuronidase from F1 C3HeB/FeJ x C57Bl/6J animals were intermediate with respect to the parental enzyme patterns, suggesting that equal concentrations of the two allelic products were present in β-glucuronidase tetramers of F1 progeny. β-glucuronidase heteropolymers assembled in vivo under conditions where equal concentrations of the two structural alleles of the enzyme were known to be present also exhibited intermediate heat-inactivation kinetics. These observations are consistent with a trans mode of action of a genetic element that controls the rate of murine β-glucuronidase synthesis.Keywords
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