Second trimester amniocentesis in twin pregnancies: maternal haemoglobin as a dye marker to differentiate diamniotic twins

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Abstract
To review the use of a membrane-free haemolysate prepared from maternal blood to distinguish the amniotic sacs at amniocentesis in twin gestation. University Hospital, Groningen. Haemoglobin solution prepared from maternal blood. 63 twin pregnancies having amniocentesis. The fetal loss before 28 weeks was 4%. There was no perinatal mortality. Dye was detected in the second sac in 9 of 24 women tested before 1985 and none of the 39 women since, no malformations could be ascribed to the use of the haemolysate. The use of the membrane-free haemolysate is safe, but the technique will probably gradually become redundant because of improved ultrasound.

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