Stable transformation of sugarbeet protoplasts by electroporation
- 1 January 1989
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 8 (2) , 71-74
- https://doi.org/10.1007/bf00716841
Abstract
Conditions were optimized for the culture, antibiotic selection and stable transformation by electroporation of suspension culture protoplasts of sugarbeet,Beta vulgaris L.. Highest plating efficiencies (up to 65% at day 21) were obtained if protoplasts were cultured in PGO salts (de Greef and Jacobs, 1979) supplemented with 0.1 mg/1 2,4-D, 0.01 mg/l BAP and 9% mannitol, and in 0.6% agarose rather than in liquid medium. Sensitivity to kanamycin also depended on whether protoplasts were cultured in liquid or agarose medium. Stable transformation of protoplast-derived colonies, as determined by resistance to kanamycin and Southern blot analysis, was achieved by electroporation using both rectangular and exponentially-decaying pulses.This publication has 13 references indexed in Scilit:
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