Specific Labelling of the Curare Binding Sites of Acetylcholinesterase and Some Properties of the Modified Enzyme

1 October 1971

journal article
Published by Canadian Science Publishing in Canadian Journal of Biochemistry

Vol. 49 (10) , 1131-1133
https://doi.org/10.1139/o71-163

Abstract

Electric eel acetylcholinesterase was found to selectively react covalently with two molecules of labelled N,N-dimethyl-2-phenylaziridinium chloride (¹⁴C-DPA) per unit of 65 000 daltons. One molecule of bound DPA is selectively labilized at pH 9.5. Both alkylated enzyme species (E.2DPA and E.DPA) are inactive toward acetylcholine as substrate, but considerably more reactive toward indophenylacetate (IPA) as substrate. The IPA activities of both the E.2DPA and E.DPA enzymes are unaffected by decamethonium bromide (C₁₀), whereas only the latter suffers inhibition by d-tubocurarine. It is concluded that the enzyme moiety where quaternary effectors interact possesses two topographically distinct classes of sites: one class is essential for C₁₀ inhibition of the esteratic activity, and the other would serve as an allosteric binding site for curare. This latter site may play the role of receptor on excitable membranes.

Keywords

This publication has 0 references indexed in Scilit: