Chemical modifications of Pokeweed antiviral protein: effects upon ribosome inactivation, antiviral activity and cytotoxicity
- 1 November 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 148 (1) , 127-130
- https://doi.org/10.1016/0014-5793(82)81257-x
Abstract
Pokeweed antiviral protein (PAP) is a protein known to inactivate eukaryotic ribosomes by an unknown enzymatic action and inhibit the production of mammalian viruses in tissue culture. This protein was subjected to a variety of chemical modifications to determine their effects upon ribosomal inactivation, antiviral action, and cytotoxicity. It was found that modifications of a number of different amino acid residues had similar effects upon all 3 activities. Also the inactivation of PAP with diethylpyrocarbonate was not due to its reaction with a histidine residue but to a modification of an unidentified amino acid residue.Keywords
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