Characteristics of tissue ultraweak chemiluminescence in rats fed with autoxidized linseed oil.

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Abstract
After 2 days of oral administration of autoxidized linseed oil (AOLO; peroxide value 1000 meq/kg, 0.3 ml/rat per day), ultraweak chemiluminescence (CL) spontaneously emitted from rat liver homogenate increased up to .apprx. 3 times accompanying the accumulation of thiobarbituric acid (TBA) reactive products. This liver CL was quenched effectively by the in vitro addition of free radical scavengers, e.g., butyl hydroxytoluene, d-.alpha.-tocopherol and 2,5-diphenylfuran, while superoxide dismutase, catalase and D-mannitol gave only negligible inhibitions on AOLO-induced liver CL. The luminescence spectrum recorded by a filter spectral analyzing method gave definitive 2 emission peaks at 585 and 635 nm and broad emission band around 500-560 nm. The spectrum was similar to that reported for a singlet molecular O2. In the experiment of successive AOLO administration (0.3 ml/rat per day) for 10 days, a large increase in CL intensities and TBA reactants in various tissues was observed, especially on the 2nd day to the 6th day of feeding. Among them, the enhancement of CL and the accumulation of TBA reactants in liver and kidney were inhibited significantly by the oral administration of supplemental d-.alpha.-tocopherol (5 mg/rat per day). AOLO-induced tissue spontaneous CL probably originated in the occurrence of free radical species which are possibly derived from chain reactions of tissue lipid peroxidation caused by the AOLO administration to rats.