DETERMINATION OF CARBON ASSIMILATION PATTERNS OF YEASTS BY REPLICA PLATING

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Abstract
The Lederbergs'' method of replica plating was used in this expt. Twenty five yeasts were inoculated in a definite pattern on a wort agar plate. After 12-24 hrs., when the colonies developed, the yeasts were transferred by the velvetine-block method to a series of plates containing washed agar, Wickerham''s N base and 0.5% of the desired C source. Colony development was recorded after 4 days and 1 wk. Five successive transfers can easily be made with 1 block. 175 yeasts, (25 authentic cultures and 150 fresh isolates from nature) representing 15 genera were tested. The results checked well with the auxano-graphic technique and the method of growth in liquid medium providing pure C sources. Some complications may arise by slow adapters to certain compounds. The advantages of the method are an enormous saving in time and the use of only 100 mg. of C source for each 25 yeasts.

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