Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis
- 1 September 2005
- journal article
- case report
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 54 (9) , 897-900
- https://doi.org/10.1099/jmm.0.46029-0
Abstract
The present report describes the diagnostic strategy followed in a case of keratomycosis. Together with conventional methods, a molecular strategy that involved the direct sequencing of an amplified portion of the genome encompassing the internal transcribed spacer 1 and 2 regions and sequence analysis was used. The data highlight the diagnostic role of molecular techniques, in parallel with conventional methods, in the management of ocular infections of fungal aetiology.Keywords
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