Purification and Characterization of a Corynebacterium ulcerans Bacteriocin (Ulceracin 378)
- 1 April 1985
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 131 (4) , 707-713
- https://doi.org/10.1099/00221287-131-4-707
Abstract
C. ulcerans strain 378 produces a bacteriocin (ulceracin 378) and a toxin when grown on semi-solid medium. Ulceracin 378 was purified 360-fold by dialysis and chromatography on DEAE-cellulose and Sephadex G-200. On the basis of Ultrogel AcA22 gel filtration its MW was .apprx. 900,000. It could be dissociated by 2-mercaptoethanol and sodium dodecyl sulfate into smaller subunits of 25,000. The bactericidal activity was associated with this subunit which contained no carbohydrate or lipid. Ulceracin 378 was thermostable and stable over a wide pH range. Purified ulceracin 378 did not have a toxic effect (lethal) on guinea pigs and rabbits and was immunologically distinct from the toxin.This publication has 2 references indexed in Scilit:
- Production of a bacteriocin, ulceracin 378, by Corynebacterium ulceransAntimicrobial Agents and Chemotherapy, 1983
- PURIFICATION OF A PROTEIN TOXIN FROM CORYNEBACTERIUM ULCERANSJournal of Medical Microbiology, 1980