Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells
Open Access
- 21 October 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 577 (3) , 345-350
- https://doi.org/10.1016/j.febslet.2004.10.027
Abstract
As an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifically targeted to the 5'-portion of the NS1 transcripts (5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an Agrobacterium strain harboring a 5mNS1-expressing binary vector caused a reduction in 5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases.Keywords
This publication has 35 references indexed in Scilit:
- RNA interference as a new biotechnological tool for the control of virus diseases in plantsVirus Research, 2004
- RNAi: an ever-growing puzzleTrends in Biochemical Sciences, 2003
- Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencingNature Biotechnology, 2003
- Gene silencing in mammals by small interfering RNAsNature Reviews Genetics, 2002
- siRNA-mediated gene silencing in vitro and in vivoNature Biotechnology, 2002
- RNA interferenceNature, 2002
- Emergence of influenza A virusesPhilosophical Transactions Of The Royal Society B-Biological Sciences, 2001
- Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegansGenes & Development, 2001
- RNAiCell, 2000
- HOW CELLS RESPOND TO INTERFERONSAnnual Review of Biochemistry, 1998