Studies in Carbohydrate Metabolism

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Abstract
Ultrafiltrates and dialysates of pig and beef plasma, whole blood and laked corpuscles were used. The solutions were concentrated at 30-37[degree]C. under reduced pressure. Those prepared from plasma were alkaline and showed dextro rotation lower than corresponded with their reducing power (Hagedorn-Jensen); if acidified to p h<4.0 the dextro rotation immediately increased but could be reversed by again rendering alkaline. Plasma was extracted with alcohol; from the extract alcohol, lipoids and pigments were removed-and sugars precipitated with CuSO4 and Ca(OH)2. The filtrate was levo-rotatory but became dextro-rotatory when acidified to p h < 4.0. Ultrafiltrates of laked corpuscles have little or no reducing power and are levo-rotatory. (The effect of acid on rotation is not stated.) No mutaro-tation was observed in any solution. The authors repeated the experiments of Lundsgaard and Holboll on glucose-muscle-insulin mixtures, and agree with Barbour in being unable to confirm the former.

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