Synthesis and Reactivity of Anthracene-1-carbonyl Azide as a Fluorescent Derivatization Reagent for Alcohols
Open Access
- 1 January 1989
- journal article
- abstracts
- Published by Pharmaceutical Society of Japan in YAKUGAKU ZASSHI
- Vol. 109 (8) , 606-610
- https://doi.org/10.1248/yakushi1947.109.8_606
Abstract
Anthracene-1-carbonyl azide was found to be a sensitive fluorescent derivatization reagent for primary and secondary alcohols for high-performance liquid chromatography. Reaction conditions were investigated for cholestanol. The reagent reacts with alcohols in benzene in the presence of 4-dimethylaminopyridine to give the corresponding fluorescent urethanes at 100 degrees C for 50 min. The detection limit for cholestanol was 250 fmol for an injection volume of 10 microliters. Tertiary alcohols and phenols do not produce fluorescent derivatives under these conditions.Keywords
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