Impregnation of Axons and Terminal Buttons in Routine, Paraffin, or Frozen Sections of Central and Peripheral Nervous Tissue Adaptation of Hortega's Silver Carbonate Method for Neurofibrils
- 1 May 1958
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 29 (5) , 449-454
- https://doi.org/10.1093/ajcp/29.5.449
Abstract
With minor adjustments, Hortega''s silver carbonate method for neurofibrils selectively impregnates axons in routine paraffin and frozen sections of formalin fixed human or experimental animal material. Using simple reagents and requiring less than 2 hr., the method works as well or better than longer, more complicated procedures. The outstanding advantage of the method is that it demonstrates normal axons remaining in degenerating fields or scars when other procedures, particularly those using current silver proteinate, may fail or provide equivocal results. Collagen, axon degeneration products, and the other background elements that impregnate have various hues of gray, pink, or purple, against which the black or purple-black axons are silhouetted; or if troublesome, background elements can be suppressed almost completely.Keywords
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