MOTILITY OF THE LIMULUS BLOOD-CELL

  • 1 January 1979
    • journal article
    • research article
    • Vol. 37  (JUN) , 169-180
Abstract
The coelomocytes of the horseshoe crab, Limulus became motile following extravasation or traumatization. Motility was studied by direct microscopic observation and time-lapse microcinematography using Nomarski optics of cells in culture and in isolated gill leaflets. In culture, solid and fluid, hydrophilic and hydrophobic surfaces were used. In all situations, motility was the same, involving the protrusion of hyaline pseudopods followed by flow of granular endoplasm forward into the pseudopods. Pseudopods often were protruded into the fluid phase and contact the substratum only after extension is complete. Motility was unaffected by antimicrotubule agents and was abolished by cytochalasin B.

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