IMMUNO-CYTOCHEMICAL LABELING OF ANAPLASMA-MARGINALE THEILER IN DERMACENTOR-ANDERSONI STILES WITH PEROXIDASE-ANTIPEROXIDASE TECHNIQUE

  • 1 January 1982
    • journal article
    • research article
    • Vol. 43  (6) , 979-983
Abstract
Colonies of A. marginale in midgut epithelial cells of adult ticks that had been infected as nymphs were specifically labeled, using the unlabeled antibody peroxidase-antiperoxidase method of immunocytochemistry. Visual comparison of infected and control tissue sections with the EM demonstrated deposition of ring-like peroxidase-antiperoxidase complexes over organisms within the colonies. The intensity of labeling differed among organisms within a single colony, possible as a result of varying antigenicity. The labeling observed on organisms in the colonies was similar to that seen on anaplasmal initial bodies in inclusions of infected bovine erythrocytes examined concurrently.

This publication has 0 references indexed in Scilit: