Maintenance and Regeneration in vitro of Bulk-Isolated Cortical Neurones from Adult Rats

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Abstract
The viability of bulk-prepared nerve cells is difficult to estimate. Metabolic studies performed immediately after the isolation procedure reflect the viability of the nerve cells only to some extent since the measured activity might be a residual one. A statement about their viability might be more meaningful if the cells could be established in culture for several days. The bulk preparation method allows the isolation of well preserved cortical nerve cells from adult rats (150-180 g), and their vitality was tested by subjecting them to culture conditions. Differentiated neurons from adult rat brain could be cultured for 2 wk. While explanted embryonic neurons begin to regenerate their fibers within the 1st day in vitro, the outgrowth of neuronal process in adult differentiated neurons is delayed for more than a week.