Abscisic acid binding to subcellular fractions from leaves of Vicia faba
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 138 (3) , 303-304
- https://doi.org/10.1007/bf00386826
Abstract
A centrifugation binding assay has been used to demonstrate the binding of [3H] (±) abscisic acid to membrane-rich fractions prepared from leaves of Vicia faba L. Kinetic analysis of this binding shows evidence of saturation of binding sites with increasing concentration of ligand. Scatchard analysis of these data yields a biphasic plot possibly indicating the presence of two types of binding sites. The dissocation constant for the high affinity site has been calculated to be 3.5×10-8 mol 1-1.This publication has 7 references indexed in Scilit:
- Characterization of Naphthaleneacetic Acid Binding to Receptor Sites on Cellular Membranes of Maize Coleoptile TissuePlant Physiology, 1977
- Separation and localization of two classes of auxin binding sites in corn coleoptile membranesPlanta, 1976
- Auxin binding to corn coleoptile membranes: Kinetics and specificityPlanta, 1976
- The Chemistry and Physiology of Abscisic AcidAnnual Review of Plant Physiology, 1974
- [36] Purification of a plasma membrane-bound adenosine triphosphatase from plant rootsPublished by Elsevier ,1974
- Inhibition by abscisic acid of the light and dark uptake of potassium by slices of Vicia faba leavesCanadian Journal of Botany, 1972
- In-vitro auxin binding to particulate cell fractions from corn coleoptilesPlanta, 1972