Fate of a Synthetic Polynucleotide Directing Cell-Free Protein Synthesis II. Association with Ribosomes
- 16 November 1962
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 138 (3542) , 813-817
- https://doi.org/10.1126/science.138.3542.813
Abstract
Tritiated-poly U, when added to cell-free extracts of Escherichia coli, became associated with ribosomes. This association occurred at 3°C and did not require high-energy phosphate compounds. Small amounts of tritiated polyuridylic acid produced polydisperse ribosomal aggregates with sedimentation constants of approximately 100 to 130. C14-phenylalanine initially was incorporated into protein only on these particles, which suggests that they are the sites of polyphenylalanine synthesis.This publication has 16 references indexed in Scilit:
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