Regulation of lipase activities in rat brain in vitro.
- 1 January 1981
- journal article
- research article
- Published by Tohoku University Medical Press in The Tohoku Journal of Experimental Medicine
- Vol. 133 (4) , 417-421
- https://doi.org/10.1620/tjem.133.417
Abstract
Regulation of acid and neutral lipase activities in rat brain was examined in vitro. Both activities were decreased by SDS [sodium dodecyl sulfate] CuCl2 and ZnCl2 and by delipidation. The neutral lipase activity was markedly reduced by N-methylmaleimide and PbCl2. The activity of delipidated preparation was increased by addition of phosphatidyl choline at both pH 5.5 and 7.5 and by phosphatidyl serine at acidic pH value. Pretreatments of the enzyme preparation with phospholipase A and trypsin reduced the lipase activities at both pH values. Phospholipids evidently play an important role on lipase activity in brain.This publication has 4 references indexed in Scilit:
- Studies on Lipase in Rat BrainJournal of Neurochemistry, 1981
- The hydrolysis of triacylglycerol and diacylglycerol by a rat brain microsomal lipase with an acidic pH optimumBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1978
- Hydrolysis of endogenous diacyglycerol and monoacylglycerol by lipases in rat brain microsomesBiochemistry, 1977
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951