Value of Heptyl-β-D-Thioglucoside, a New Nonionic Detergent, in Studies on Membrane Proteins
- 1 June 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 97 (6) , 1807-1810
- https://doi.org/10.1093/oxfordjournals.jbchem.a135240
Abstract
A new nonionic detergent, heptylthioglucoside, was synthesized and found to be more soluble than octylthioglucoside in water at low temperatures. Use of this detergent for solubilization and reconstitution of membrane proteins of Escherichia coli was examined. Heptylthioglucoside was as effective as octylthioglucoside and octylglucoside in solubilizing membrane proteins, and by the heptylthioglucosidedilution procedure the H+-translocating ATPase (F1F0) and melibiose carrier could easily be reconstituted into liposomes. It is concluded that heptylthioglucoside is very useful in studies on membrane proteins.This publication has 2 references indexed in Scilit:
- Characteristics of n-octyl β-d-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistryBiochemical Journal, 1984
- Solubilization and Reconstitution of Membrane Proteins of Escherichia coli Using Alkanoyl-N-Methylglucamides1The Journal of Biochemistry, 1984