The binding of cationic detergent to insulin, zinc–insulin and iso-insulin (Short Communication)
- 1 September 1973
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 135 (1) , 253-255
- https://doi.org/10.1042/bj1350253
Abstract
Insulin, zinc–insulin and iso-insulin were precipitated by cetyltrimethylammonium bromide when the detergent/protein molar ratio was less than 10:1, whereas no precipitation was observed at ratios greater than 10:1. It is concluded that the detergent binds as a dimer at each binding site at ratios greater than 10:1. U.v. difference spectra indicated that the tyrosine residues were perturbed by detergent binding.Keywords
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- Hydrogen Ion Equilibria in Native and Denatured ProteinsPublished by Elsevier ,1955