BETA-ADRENERGIC-RECEPTOR SUBTYPES IN THE ATRIA - EVIDENCE FOR CLOSE COUPLING OF BETA-1 AND BETA-2 ADRENERGIC-RECEPTORS TO ADENYLATE-CYCLASE

  • 1 September 1986
    • journal article
    • research article
    • Vol. 238  (3) , 886-892
Abstract
The relationship between occupancy of beta adrenergic receptors and stimulation of adenylate cyclase in dog atrial tissue was examined by studying the binding of [125I]iodopindolol and the activation of adenylate cyclase. Computer-assisted nonlinear regression analysis was used to analyze the inhibition of isoproterenol-stimulated adenylate cyclase activity by beta-1- or beta-2-selective antagonists. The Ki values for each subtype of receptor for the selective antagonists resulting from studies of the inhibition of adenylate cyclase activity were similar to those determined in studies of the inhibition of the binding of [125I] iodopindolol. To compare further the occupancy of beta-1 or beta-2 adrenergic receptors with the activation of adenylate cyclase mediated by each class of receptor, computer modeling of the stimulation of adenylate cyclase by the beta-1-selective agonist norepinephrine was carried out. The EC50 values of norepinephrine for each receptor subtype, as measured in studies of norepinephrine-stimulated adenylate cyclase activity, were similar to the Ki values for the inhibition by norepinephrine of the binding of [125I]iodopindolol to each receptor subtype. The data led to the conclusion that beta-1 adrenergic receptors make up about 70% of the total number of beta adrenergic receptors and mediate 70% of the increase in adenylate cyclase activity produced by isoproterenol. These results suggest that the relationship between occupancy of each class of receptor and activation of adenylate cyclase is linear and that, when agonist-stimulated adenylate cyclase activity is used as a functional response, neither spare beta-1 nor spare beta-2 adrenergic receptors exist in the atrium. Finally, a bromo-acetylated derivative of alprenolol, Alm-CO-CH2Br, was used to inactivate irreversibly beta adrenergic receptors in dog atria. In atrial membranes pretreated with different concentrations of Alm-CO-CH2Br, a decrease in receptor density correlated with a reduction in the maximal level of isoproterenol-stimulated adenylate cyclase activity. These experiments provide direct evidence that all of the beta adrenergic receptors in the atria are coupled to adenylate cyclase.