THE EFFECT OF INCUBATION TIME ON MACROCOLONY FORMATION AFTER X-IRRADIATION OF MICROORGANISMS

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Abstract
Washed cells suspended in K phosphate buffer were plated on yeast agar or yeast-dextrose agar either directly or after irradiation with varying doses of 250 kv X-rays. Macrocolonies were counted at intervals up to 11 days at 30[degree]C. With unirradiated and irradiated Escherichia coli (strain W-1485), Pseudomonas fluorescens Azotobacter agile Rhodopseudomonas spheroides and Saccharomyces cerevisiae (haploid), and unirradiated S. cerevisiae (diploid), the viable counts did not change following the initial appearance of visible colonies. However, irradiated S. cerevisiae (diploid) showed increases in viable counts for a period of 2 - 3 days following the first appearance of visible colonies. The magnitude of this increase was a function of the dose of radiation.

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