INVITRO ERYTHROPOIESIS .2. CYTOCHEMICAL ENUMERATION OF ERYTHROID STEM-CELLS (CFU-E AND BFU-E) FROM NORMAL MOUSE AND HUMAN HEMATOPOIETIC TISSUES

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 7  (5) , 219-224
Abstract
A procedure which provides more accuracy in the identification and scoring of in vitro derived erythroid colonies (i.e., BFU-e [erythropoietic burst-forming units] and CFU-e [erythroid colony-forming units] from normal mouse and human marrow and spleen cells was developed. Up to now identification of erythroid colonies was based on staining with acidified benzidine which consistently displays background staining of granulocyte/macrophage colonies. The staining reaction with these reagents is such that identification of erythroid colonies is less than precise. The modified benzidine procedure described eliminates the staining reactivity of granulocyte/macrophage colonies and specifically reacts with only those cells that contain Hb. Individual colonies and/or cells may readily be counterstained and examined by high resolution light microscopy.

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