A collagenase in extracts of the invertebrate Bipalium kewense
- 1 June 1973
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 133 (2) , 329-334
- https://doi.org/10.1042/bj1330329
Abstract
The localization, isolation and partial characterization of a collagenolytic enzyme from the land planarian Bipalium kewense is described. This enzyme can be obtained by direct extraction of the organism, and can be separated from non-collagenous proteolytic activity by (NH4)2SO4 precipitation and Sephadex-gel chromatography. Its mode of attack on collagen and sensitivity to a variety of inhibitors indicate that this enzyme differs from vertebrate collagenases and a previously described invertebrate collagenase.Keywords
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