Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 170 (2) , 161-169
- https://doi.org/10.1007/bf00337792
Abstract
Bacteriophage lambda vectors, derived from λplac5 were constructed. Their genomes have only one EcoRI restriction site, located near the end of the β-galactosidase gene. Recombinants, constructed in vitro, having a DNA fragment inserted in the EcoRI site, are lac - and can be easily recognized. Expression of such foreign genes is then under the control of the lac promoter. Mutations Qam73 and Sam7 greatly increase the amount of β-galactosidase synthesized by the vector bacteriophage. The λZEQS vector has been certified B2 (EK2) by the French control commission “Recombinaisons génétiques in vitro”.This publication has 35 references indexed in Scilit:
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