An optical biosensor based upon glucose oxidase immobilized in sol‐gel silicate matrix
- 1 June 1994
- journal article
- Published by Wiley in Biotechnology and Applied Biochemistry
- Vol. 19 (3) , 293-305
- https://doi.org/10.1111/j.1470-8744.1994.tb00299.x
Abstract
Glucose oxidase immobilized in a transparent silicate gel prepared by a sol‐gel method was used as a simple solid‐state optical biosensor for glucose. The sensor was based upon the measurement of initial rates of reduction of the FAD prosthetic group of the enzyme in the presence of various concentrations of glucose. The analytical range of the sensor was 1‐100 mM, and the measurement time was 2 min. The enzyme was considerably protected by the silicate matrix against leaching, thermal inactivation and even H2O2‐dependent inactivation. The sensor was stable in daily use for 6 months.This publication has 0 references indexed in Scilit: