Observations on the Hemagglutination of Immature Normoblasts

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Abstract
Normoblasts from a case of erythromyelosis maligna (Di Guglielmo''s disease) were studied for their ability to agglutinate. Influenza virus and cold agglutlnlns were used as agglutinating agents. The cells were found to agglutinate to the same extent as mature erythrocytes exposed to these agents. It was also shown that the cells increased their vol. in hypotonic salt soln., some cells then assuming a structure similar to that of the megaloblast. It is concluded that these primitive erythro-blasts already have membranes of the same chemical composition as the mature erythrocyte. As the morphological evidence supports the view that these cells are primitive it is suggested that the methods described should be used in order to differentiate stem cell leukemias.