Die Kreismessermethode, ein neues Verfahren zum Schneiden tierischer und pflanzlicher Stoffe

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Abstract
The apparatus for preparing tissue slices is made from an old sliding microtome, the knife being a circular saw blade, 8-15 cm. in diam. The tissue is fastened to a cork with gelatin, and is moved against the rotating circular knife. The thickness of the tissue cut is controlled by a micrometer screw. The lower part of the knife passes through a Petri dish filled with Kreb''s nutrient soln., in which the tissue slices are collected. Tissue slices as little as 0.2 mm. in thickness can be cut without being torn or crushed. From the slices, circular pieces of the desired size can be cut with cork borers, and the volume of tissue used in an expt. thus readily calculated. The respiration of the tissues is defined by the equation: [image] The wt. of the tissue used is detd. by multiplying the tissue vol. by the specific wt. of the organ. About 100 mg. of rat, kidney, liver, spleen, etc., are used for adetn. The absorp-tion flask contains 50% KOH, and the expts. are carried out 1-12 hrs. For each tissue, the same number of slices, having the same thickness and the same surface area, use the same amt. of O2 during the same time. In expts. in which slices of the same thickness but with a surface area in the ratio 1:2:3 are used, the O2 consumption increases in proportion to the surface area, but QO2 remains constant. In respiration expts. of 8-12 hrs. duration, it is better to use tissue slices 0.5-0.6 mm. in thickness, for the O2 quotient decreases more rapidly with thin than with thick slices.

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