Accurate quantitation of standard peptides used for quantitative proteomics
- 7 August 2009
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 9 (15) , 3939-3944
- https://doi.org/10.1002/pmic.200900043
Abstract
MS‐based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC‐MS/MS (UPLC‐MS/MS) to accurately quantify peptide standards at concentrations of 0.1–10 μM. The ability for accurate quantitation of micro‐molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.Keywords
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