Liposome destabilization induced by the HIV‐1 fusion peptide Effect of a single amino acid substitution
- 3 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 362 (2) , 243-246
- https://doi.org/10.1016/0014-5793(95)00257-a
Abstract
The 23-residue synthetic peptide representing the N-terminus of HIV-1 gp41 is known to induce either leakage or fusion of lipid vesicles depending on the experimental conditions. In this paper we report that a polar amino acid substitution V → E at position 2, known to block gp41 activity in vivo, makes the peptide unable to destabilize and/or fuse membranes. Moreover this variant, unlike the parent peptide, is never found in the membrane-associated β conformationKeywords
This publication has 20 references indexed in Scilit:
- Production of large unilamellar vesicles by a rapid extrusion procedure. Characterization of size distribution, trapped volume and ability to maintain a membrane potentialPublished by Elsevier ,2002
- Membrane Interaction and Conformational Properties of the Putative Fusion Peptide of PH-30, a Protein Active in Sperm-Egg FusionBiochemistry, 1994
- Interaction of the HIV-1 Fusion Peptide with Phospholipid Vesicles: Different Structural Requirements for Fusion and LeakageBiochemistry, 1994
- Peptide models for the membrane destabilizing actions of viral fusion proteinsBiopolymers, 1992
- Investigation of Human Immunodeficiency Virus Fusion Peptides. Analysis of Interrelations Between Their Structure and FunctionAIDS Research and Human Retroviruses, 1992
- Membrane fusion activity of the influenza virus hemagglutinin: interaction of HA2 N-terminal peptides with phospholipid vesiclesBiochemistry, 1991
- Fusogenic activity of SIV (Simian Immunodeficiency Virus) peptides located in the GP32 NH2 terminal domainBiochemical and Biophysical Research Communications, 1991
- An infrared spectroscopic study of β‐galactosidase structure in aqueous solutionsFEBS Letters, 1989
- Detection of a fusion peptide sequence in the transmembrane protein of human immunodeficiency virusCell, 1987
- A rapid and sensitive sub-micro phosphorus determinationAnalytica Chimica Acta, 1961