Inositol Trisphosphate Mobilizes Intracellular Calcium in Permeabilized Adrenal Chromaffin Cells
- 1 February 1986
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 46 (2) , 637-640
- https://doi.org/10.1111/j.1471-4159.1986.tb13014.x
Abstract
Using permeabilized chromaffin cells and the fluorescent probe Quin 2 (an indicator of free Ca2+), we found that inositol trisphosphate (IP3) specifically triggered an immediate and dose-dependent release of Ca2+ from intracellular stores. Desensitization of the response was observed at nonsaturating concentrations of inositol trisphosphate and resequestration of Ca2+ was not observed. While representing only a small fraction of the total cellular Ca2+, the amount released by IP3 could significantly raise cytosolic Ca2+ and may acount for muscarinic effects on Ca2+ metabolism in chromaffin cells.Keywords
This publication has 22 references indexed in Scilit:
- Inositol(1,4,5)trisphosphate-promoted Ca2+ release from microsomal fractions of rat liverBiochemical and Biophysical Research Communications, 1984
- The second messenger linking receptor activation to internal Ca release in liverNature, 1984
- Effects of changes in osmolality on the stability and function of cultured chromaffin cells and the possible role of osmotic forces in exocytosisThe Journal of cell biology, 1983
- EFFECT OF MUSCARINE ON RELEASE OF CATECHOLAMINES FROM THE PERFUSED ADRENAL GLAND OF THE CATBritish Journal of Pharmacology, 1982
- Relationship Between Ca2+ Uptake and Catecholamine Secretion in Primary Dissociated Cultures of Adrenal MedullaJournal of Neurochemistry, 1982
- Calcium Uptake and Catecholamine Secretion by Cultured Bovine Adrenal Medulla CellsJournal of Neurochemistry, 1982
- Muscarinic Receptors in Chromaffin Cell Cultures Mediate Enhanced Phospholipid Labeling but Not Catecholamine SecretionJournal of Neurochemistry, 1981
- Phosphatidylinositol Labelling in Response to Activation of Muscarinic Receptors in Bovine Adrenal MedullaJournal of Neurochemistry, 1981
- Possible muscarinic regulation of catecholamine secretion mediated by cyclic GMP in isolated bovine adrenal chromaffin cellsBiochemical Pharmacology, 1981
- The role of calcium in the secretory response of the adrenal medulla to acetylcholineThe Journal of Physiology, 1961