MALDI-MS and HPLC Quantification of Oligosaccharides of Lichenase-Hydrolyzed Water-Soluble β-Glucan from Ten Barley Varieties

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Abstract
This study is the first to apply matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to both qualitative and quantitative analyses of oligosaccharides of lichenase-hydrolyzed water-soluble β-glucan from barley. Compared to high-performance liquid chromatography (HPLC) with an evaporative light-scattering detector, MALDI-MS is a rapid technique with high accuracy and sensitivity and could be used to assess primary structural features of water-soluble β-glucan from different barley varieties. Keywords: Barley; β-glucan; MALDI-MS; oligosaccharides; quantification