Host cell protein tyrosine kinases are activated during the entry ofListeria monocytogenes
Open Access
- 1 May 1998
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 162 (1) , 169-176
- https://doi.org/10.1111/j.1574-6968.1998.tb12995.x
Abstract
Listeria monocytogenes is able to invade a wide range of cell types by inducing its own internalization. Little is known, however, about the host cell proteins affecting the entry process which involves triggering the host cell signal transduction mechanism. We report here that entry of L. monocytogenes strains (serotypes 4b and 1/2a) into Caco-2 cells induced tyrosine phosphorylation of several host cell proteins including pp60c-src substrates. Using specific synthetic peptide substrates, we showed that L. monocytogenes activates, as early as 5 min after bacteria-cell contact, the pp60c-src family-related (srcFR) proteins by an inlAB-dependent pathway. The activation of srcFR proteins seems to be crucial in the entry of L. monocytogenes into Caco-2 cells. Indeed, specific inhibition of the srcFR signal by herbimycin A blocked the entry of L. monocytogenes strains. Taken together, our data show that L. monocytogenes enhances cell tyrosine phosphorylations and activates the pp60c-src family-related proteins by an inlAB-dependent pathway.Keywords
This publication has 11 references indexed in Scilit:
- Cell proliferation enhances entry of Listeria monocytogenes into intestinal epithelial cells by two proliferation-dependent entry pathwaysJournal of Medical Microbiology, 1997
- Exploitation of Mammalian Host Cell Functions by Bacterial PathogensScience, 1997
- Listeria monocytogenes infection of HeLa cells results in listeriolysinO-mediated transient activation of the Raf-MEK-MAP kinase pathwayFEMS Microbiology Letters, 1997
- A Role for Phosphoinositide 3-Kinase in Bacterial InvasionScience, 1996
- E-Cadherin Is the Receptor for Internalin, a Surface Protein Required for Entry of L. monocytogenes into Epithelial CellsCell, 1996
- V-src kinase shifts the cadherin-based cell adhesion from the strong to the weak state and beta catenin is not required for the shift.The Journal of cell biology, 1995
- Entry of Listeria monocytogenes into hepatocytes requires expression of InIB, a surface protein of the internalin multigene familyMolecular Microbiology, 1995
- DNA Synthesis Induced by Some but Not All Growth Factors Requires Src Family Protein Tyrosine KinasesMolecular and Cellular Biology, 1995
- Protein tyrosine kinase inhibitors block the entries of Listeria monocytogenes and Listeria ivanovii into epithelial cellsMicrobial Pathogenesis, 1994
- Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocciCell, 1991