Induction of plasma membrane alkaline phosphatase in rat liver
- 1 April 1985
- journal article
- research article
- Published by Wiley in Cell Biochemistry and Function
- Vol. 3 (2) , 95-100
- https://doi.org/10.1002/cbf.290030204
Abstract
We have determined alkaline phosphatase activity in total liver plasma membrane fractions from rats subjected to a partial hepatectomy and sham operated with or without manipulation of the liver. In all these cases, an increase of the enzyme activity was observed. Kinetic studies of alkaline phosphatase activity performed on plasma membrane fractions from rats subjected to a partial hepatectomy suggest that alkaline phosphatase increase is produced by de novo biosynthesis of enzyme molecules. Determination of alkaline phosphatase activity in purified plasma membrane subfractions corresponding to each of the three functional regions of the hepatocyte surface (blood sinusoidal, lateral and bile canalicular), indicates that the increase of the enzyme activity observed after partial hepatectomy is selectively induced in the bile canalicular domain of the hepatocyte plasma membrane.Keywords
This publication has 17 references indexed in Scilit:
- Quantitative and qualitative changes of plasma membrane glycoproteins in the early period of liver regenerationCell Biochemistry and Function, 1984
- A biochemical dissection of the functional polarity of the plasma membrane of the hepatocyteBiochimica et Biophysica Acta (BBA) - Biomembranes, 1980
- Improve method for isolation of rat liver plasma membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- [6]Isolation of rat liver plasma membrane fragments in isotonic sucrosePublished by Elsevier ,1974
- Alteration of Bile Canalicular Enzymes in Cholestasis. A POSSIBLE CAUSE OF BILE SECRETORY FAILUREJournal of Clinical Investigation, 1973
- Increases in rat liver cyclic AMP concentrations prior to the initiation of DNA synthesis following partial hepatectomy or hormone infusionBiochemical and Biophysical Research Communications, 1972
- Sheep brain 5'-nucleotidase. Enzymic properties and allosteric inhibition by nucleoside triphosphatesBiochemistry, 1968
- THE ISOLATION AND PROPERTIES OF CARDIAC RIBOSOMES AND POLYSOMESBiochemical Journal, 1965
- [29] PhosphodiesterasesPublished by Elsevier ,1963
- [83] Glucose-6-phosphatase from liverPublished by Elsevier ,1955