Comparative Chromosomal Studies on Mammalian Cells in Culture. II. Mouse Sarcoma-Producing Cell Strains and Their Derivatives2

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Abstract
Comparative cytological studies were made of mouse sarcoma-producing cell lines maintained in different media, their clonal derivatives, and the sarcomas produced by injection of these lines into mice of the original strain. These sublines were derived from one cell cultured from a cell strain that originated in the normal tissue of a mouse, but differences in morphology, metabolism, and in the frequency with which they produce sarcomas were demonstrated. Mitotic rate comparisons showed that all cell lines exhibited similar mitotic activities in vitro. In addition, mitotic abnormalities were observed in all lines. These lines may be classified as hyperdiploids, with most lines exhibiting characteristic modal chromosome numbers. Variable proportions of higher polyploid cells were found in all lines. Metacentric chromosomes are present in all lines, ranging on the average from 2 to 11 per cell in each line. Furthermore, certain characteristic marker chromosomes can be identified in individual lines. These can be detected in related strains and in the resulting sarcomas. These cell strains clearly have altered chromosome number and morphology as compared with normal mouse cells. Although these studies have established certain characteristic chromosomal differences between the high and low sarcoma-producing lines, there is no present evidence that these differences are directly responsible for their distinctive physiological properties.

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