A jumping gene in Streptomyces coelicolor A3(2)

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Abstract
Summary The difficulty in mapping the gene for chloramphenicol resistance (cml R) in Streptomyces coelicolor A3(2) stock strains is possibly due to its location on different sites of the chromosome in various mixed subelones. Fresh isolates from CmlR strains show single unequivocal locations of cml R. The same holds for CmlR strains derived as revertants from CmlS variants. The two best established sites for cml R are one between cys A and met A, the other at right of arg A, possibly in the right empty arc of the map (Fig. 2). The cml R gene was assumed to be on a transposon (SCTn1), together with a gene for arginine-succinate synthase (argG), a gene for chromosome transfer (tra) and a gene for aereal mycelium formation (amy). In a CmlR revertant, the cml R gene appears disjoined from argG (Fig. 5), thus showing the ability of SCTnl to be split and partially transposed. The possible wide occurrence of transposons in the genus Streptomyces is discussed.