The linear tetrasaccharide, Gal?1-4GlcNAc?1-6Gal?1-4GlcNAc, isolated from radiolabeled teratocarcinoma poly-N-acetyllactosaminoglycan resists the action ofE. freundii endo-?-galactosidase

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Abstract
A novel linear tetrasaccharide, Galβ1-4GlcNAcβ1-6Galβ1-4GlcNAc, was isolated from partial acid hydrolysates of metabolically labeled poly-N-acetyllactosaminoglycans of murine teratocarcinoma cells. It was characterized by exo-glycosidase sequencing and by mild acid hydrolysis followed by identification of all partial cleavage products. The tetrasaccharide, and likewise labelled GlcNAcβ1-6Galβ1-4GlcNAc, resisted the action of endo-β-galactosidase (EC 3.2.1.103) fromE. freundii at a concentration of 125 mU/ml, while the isomeric, radioactive teratocarcinoma saccharides Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAc and GlcNAcβ1-3Galβ1-4GlcNAc were cleaved in the expected manner.