Histochemical staining of the acetylcholine receptor, acetylcholinesterase, and the axon terminal

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Abstract
Fluoresceinated alpha‐bungarotoxin (α‐BT) and the Pestronk‐Drachman neuromuscular stain were combined to demonstrate the acetylcholine receptor, the junctional acetylcholinesterase, and the nerve terminal on the same endplate. The method is reliable and rapid. Synapses in both normal muscle and muscle affected by amyotrophic lateral sclerosis (ALS) are equally well displayed.