Inhibition by forskolin of cytosolic calcium rise, shape change and aggregation in quin2‐loaded human platelets
- 19 August 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 188 (1) , 135-140
- https://doi.org/10.1016/0014-5793(85)80890-5
Abstract
The adenylate cyclase stimulator forskolin was used to study the inhibitory effects of elevated cAMP on the activation of washed human platelets loaded with the fluorescent Ca2+ indicator quinl. In the presence of 10 μM isobutylmethylxanthine forskolin inhibited rises in [Ca2+]i evoked by thrombin and platelet-activating factor (PAF) due to both Ca2+ influx and release from internal stores with similar potency. Aggregation evoked by thrombin and PAF was suppressed whilst partial shape-change persisted, even in the absence of a measurable rise in [Ca2+]i. Forskolin did not affect the rise in [Ca2+i evoked by Ca2+ ionophore; aggregation was suppressed but shape-change persistedKeywords
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