A novel cell-ablation strategy for studying plant development

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Abstract
The processes controlling the differentiation of plant cells are not well understood. Two alternative, but not mutually exclusive, mechanisms probably play a major role in plant-cell differentiation. One mechanism utilizes a position-independent, cell-autonomous differentiation process. The other, employs a position-dependent, cell-cell interaction process that requires signals from neighbouring cells. Cell ablation studies can be used to distinguish between these two models of plant-cell differentiation. In this article we outline a new cell-ablation strategy that utilizes promoters with distinct, but overlapping, cell specificities that are fused with cytotoxic and anticytotoxic structural genes. We present preliminary observations on how this strategy can be used to dissect the events controlling anther development.