Determination of morphine in body fluids by high-performance liquid chromatography with chemiluminescence detection
- 1 January 1987
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 112 (4) , 397-406
- https://doi.org/10.1039/an9871200397
Abstract
HPLC has been used in combination with chemiluminescence detection for the determination of morphine in biological fluids. Solid-liquid extraction procedures for the isolation of morphine from whole blood and urine are described, as is the synthesis of the internal standard, N-ethylnormorphine. Improved selectivity and sensitivity over UV detection is apparent. The procedure has a detection limit of 5 ng of morphine on-column after extraction from blood or urine. The utility of the procedure has been tested on forensic case samples.Keywords
This publication has 0 references indexed in Scilit: